Browsing by Subject "qPCR"
Now showing items 1-6 of 6
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Development of a quantitative assay for the non-invasive determination of fetal sex in Sable and Roan antelope
(North-West University (South Africa), 2021)The research field of cell free DNA (cfDNA) and cell free fetal DNA (cffDNA) has expanded in the past decade, with the inclusion of animal based cfDNA studies. This has resulted in an increase in research making use of ... -
Development of a real time PCR assay to distinguish between Coxiella burnetii and Coxiella-like endosymbionts
(North-West University (South Africa), 2023)Q fever is classified as notifiable by the World Organisation of Animal Health (OIE, now known as the World Organisation for Animal Health, WOAH). It is a zoonotic bacterial disease caused by Coxiella burnetii that occurs ... -
The dynamics of toxic microcystis strains and microcystin production in two hypertrofic South African reservoirs
(Elsevier, 2012)The South African impoundments of Hartbeespoort and Roodeplaat experience excessive blooms of Microcystis species each year. Microcystins, produced primarily by strains of cyanobacteria belonging to the genera Microcystis, ... -
The ecology of chytrid lineages in southern Africa
(North-West University (South Africa), 2020)The inter- and intraspecific diversity of microbial communities is known to be an important, but difficult to disentangle, factor in pathogen ecology. Conspecific or interspecific microbial interactions may result in ... -
Improvement of diagnostics for Trypanosoma equiperdum infecting equines in South Africa
(North-West University (South Africa). Potchefstroom Campus, 2019)Dourine is a sexually transmitted disease of equines caused by Trypanosoma equiperdum. Dourine has worldwide distribution and is an economically important veterinary disease. There is little to no active research on dourine ... -
A quantitative assessment of cell-free DNA utilizing several housekeeping genes: measurements from four different cell lines
(Springer, 2016)Quantitative real-time PCR (qPCR) is regularly used to quantify cell-free nucleic acids (cfNAs) in order to identify biomarkers for various pathologies. However, studies have shown notable housekeeping gene expression ...