Cytotoxic effects of polystyrene–titanium–arsenate composite in cultured H9c2 cardiomyoblasts
Abstract
In the present report, we explored the toxicological
behaviour of engineered polystyrene–titanium–
arsenate (PS–Ti–As) composite using cultured H9c2 cardiomyoblasts
in vitro. From in vitro cytotoxicity studies, it
appears that the composite can be toxic to the cardiac cells
and the value of IC50 investigated to be the highest concentration
of 500 lg mL-1, during 16–24 h of incubation
period. The cell morphological studies based on dual
staining with acridine orange and ethidium bromide indicates
that apoptosis is the dominating pathway of cell
death. Furthermore, an enhanced DNA fragmentation,
increased reactive oxygen species production and caspase
release demonstrates the potential risks associated with the
exposure of PS–Ti–As composite to the cardiac cells.