dc.contributor.author | Du Plessis, Lissinda H. | |
dc.contributor.author | Hamman, Josias H. | |
dc.date.accessioned | 2015-10-21T10:14:20Z | |
dc.date.available | 2015-10-21T10:14:20Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Du Plessis, L.H. & Hamman, J.H. 2014.In vitro evaluation of the cytotoxic and apoptogenic properties of aloe whole leaf and gel materials. Drug and chemical toxicology, 37(2):169-177. [https://doi.org/10.3109/01480545.2013.834356] | en_US |
dc.identifier.issn | 0148-0545 | |
dc.identifier.issn | 1525-6014 (Online) | |
dc.identifier.uri | http://hdl.handle.net/10394/14842 | |
dc.identifier.uri | https://www.tandfonline.com/doi/full/10.3109/01480545.2013.834356 | |
dc.identifier.uri | https://doi.org/10.3109/01480545.2013.834356 | |
dc.description.abstract | Aloe gel and whole-leaf materials have shown biological effects with potential therapeutic
applications, and recently, their drug-absorption enhancement properties have been discovered.
It is important to establish a safety profile for these materials before they can be used
in pharmaceutical products. The aim of the study was to investigate the in vitro cytotoxicity of
Aloe vera, Aloe marlothii, Aloe speciosa and Aloe ferox against human hepatocellular (HepG2),
human neuroblastoma cells (SH-SY5Y) and human adenocarcinoma epithelial cells (HeLa). Flow
cytometry was used to measure cell viability, apoptosis and reactive oxygen species (ROS). The
aloe gel materials investigated only decreased cell viability at concentrations of410 mg/mL
and exhibited half-maximal cytotoxic concentration (CC50) values above 1000 mg/mL, except
for A. vera gel in HepG2 cells (CC50¼269.3 mg/mL). A. speciosa whole-leaf material showed
a significant decrease in viability of Hela cells, whereas the other whole-leaf materials did not
show a similar effect. The aloe gel materials in general showed low levels of apoptosis, whereas
A. vera and A. speciosa whole-leaf materials caused a dose-dependent increase of apoptosis
in HeLa cells. None of the aloe materials investigated exhibited a significant increase in ROS.
It can be concluded that the selected aloe materials caused only limited reduction in cell
viability with limited in vitro cytotoxicity effects. Further, neither significant apoptosis effects
were observed nor induction of ROS. | en_US |
dc.description.sponsorship | USA Inc. (DeSoto, TX) and Organic Aloe (Albertinia, South Africa) | en_US |
dc.language.iso | en | en_US |
dc.publisher | Taylor & Francis | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | A. ferox | en_US |
dc.subject | A. marlothii | en_US |
dc.subject | A. speciosa | en_US |
dc.subject | A. vera | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Reactive oxygen species | en_US |
dc.title | In vitro evaluation of the cytotoxic and apoptogenic properties of aloe whole leaf and gel materials | en_US |
dc.type | Article | en_US |
dc.contributor.researchID | 11948388 - Du Plessis, Lissinda Hester | |
dc.contributor.researchID | 10081097 - Hamman, Josias Hendrik | |