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dc.contributor.advisorDu Plessis, J.
dc.contributor.advisorGrobler, A.F.
dc.contributor.authorCoetzee, Hanneri
dc.date.accessioned2009-03-16T08:51:30Z
dc.date.available2009-03-16T08:51:30Z
dc.date.issued2007
dc.identifier.urihttp://hdl.handle.net/10394/1491
dc.descriptionThesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2007.
dc.description.abstractThe aim of this study was to investigate in vitro transdermal diffusion of a small peptide namely arginine vasopressin (AVP) with the aid of the novel PheroidTM drug delivery system. Generally, peptides seem unfit for transdermal permeation, but it was thought prudent to explore the suitability of this lipid-based system after success was achieved with entrapment of tuberculostatics, bacteria and viruses. Bestatin (a selective aminopeptidase inhibitor) was employed to circumvent any skin-related degradation of the active. Therefore, the effect of bestatin on the preservation of AVP during diffusion was investigated. Vertical Franz cell diffusion studies were conducted with female abdominal skin, with AVP at a concentration of 150 µg/ml in the donor phase and Hepes buffer as the receptor phase over a twelve-hour period. To prove entrapment of AVP within the lipid structures of the PheroidsTM, fluorescentlylabelled samples were monitored by means of confocal laser scanning microscopy (CLSM), which revealed definite entrapment. In vitro permeation profiles for AVP exhibited a biphasic character, with the majority of permeation occurring during the first two hours. The PheroidTM delivery system proved to be advantageous when applied as delivery medium. The inclusion of bestatin has an enhancing effect on permeation probably due to its protection of AVP.
dc.publisherNorth-West University
dc.subjectArginine vasopressinen
dc.subjectTransdermal diffusionen
dc.subjectConfocal microscopyen
dc.subjectPheroiden
dc.subjectDelivery systemen
dc.subjectBestatinen
dc.titleThe transdermal delivery of arginine vasopressin with pheroid technologyen
dc.typeThesisen
dc.description.thesistypeMasters


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