dc.contributor.author | Liebenberg, Nico | |
dc.contributor.author | Wegener, Gregers | |
dc.contributor.author | Joca, Sâmia | |
dc.date.accessioned | 2016-08-22T06:54:37Z | |
dc.date.available | 2016-08-22T06:54:37Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Liebenberg, N. et al. 2015. Nitric oxide involvement in the antidepressant-like effect of ketamine in the Flinders sensitive line rat model of depression. Acta neuropsychiatrica, 27(2):90-96. [https://doi.org/10.1017/neu.2014.39] | en_US |
dc.identifier.issn | 0924-2708 | |
dc.identifier.issn | 1601-5215 (Online) | |
dc.identifier.uri | http://hdl.handle.net/10394/18346 | |
dc.identifier.uri | https://doi.org/10.1017/neu.2014.39 | |
dc.description.abstract | Objective: We investigated whether the nitric oxide (NO) precursor,
L-arginine, can prevent the antidepressant-like action of the fast-acting
antidepressant, ketamine, in a genetic rat model of depression, and/or
induce changes in the glutamate (Glu)/N-methyl-D-aspartate receptor
(NMDAR)/NO/cyclic guanosine monophosphate (cGMP) signalling
pathway. Hereby it was evaluated whether the NO signalling system is
involved in the antidepressant mechanism of ketamine.
Methods: Flinders sensitive line (FSL) rats received single i.p. injections
of ketamine (15 mg/kg) with/without pre-treatment (30 min prior) with
L-arginine (500 mg/kg). Depression-like behaviour was assessed in the
forced swim test (FST) in terms of immobility, and the activation state of
the Glu/NMDAR/NO/cGMP pathway was evaluated ex vivo in the frontal
cortex and hippocampus regions in terms of total constitutive NOS
(cNOS) activity and cGMP concentration.
Results: L-Arginine pre-treatment prevented the antidepressant-like effect
of ketamine in the FST, as well as a ketamine-induced increase in cGMP
levels in the frontal cortex and hippocampus of FSL rats. Ketamine
reduced cNOS activity only in the hippocampus, and this effect was not
reversed by L-arginine.
Conclusion: Both the behavioural and molecular results from this study
indicate an involvement for the NO signalling pathway in the
antidepressant action of ketamine. Although not easily interpretable, these
findings broaden our knowledge of effects of ketamine on the NO
system | en_US |
dc.description.sponsorship | Danish Medical
Research Council (grant 11-107897) and the AUIDEAS
initiative (eMOOD) | en_US |
dc.language.iso | en | en_US |
dc.publisher | Cambridge Univ Press | en_US |
dc.subject | Flinders sensitive line rats | en_US |
dc.subject | NOS activity | en_US |
dc.subject | Antidepressant | en_US |
dc.subject | Ketamine | en_US |
dc.subject | Nitric oxide | en_US |
dc.title | Nitric oxide involvement in the antidepressant-like effect of ketamine in the Flinders sensitive line rat model of depression | en_US |
dc.type | Article | en_US |
dc.contributor.researchID | 22353003 - Wegener, Gregers | |