Browsing Faculty of Health Sciences by Author "Grobler, Anne"
Now showing items 1-19 of 19
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99mTc-MDP as an imaging tool to evaluate the in vivo biodistribution of solid lipid nanoparticles
Mandiwana, Vusani; Grobler, Anne; Zeevaart, Jan Rijn; Kalombo, Lonji (Elsevier, 2018)The aim of this study was to establish the in vivo uptake and tissue distribution of 99mTc-MDP-encapsulated Solid Lipid Nanoparticles (SLNs) post administration. Radioactive 99mTc-MDP encapsulated into SLNs was administered ... -
Bioaccumulation and subchronic toxicity of 14 nm gold nanoparticles in rats
Rambanapasi, Clinton; Zeevaart, Jan Rijn; Buntting, Hylton; Bester, Cornelius; Hayeshi, Rose; Grobler, Anne (MDPI, 2016)Colloidal suspensions of 14 nm gold nanoparticles (AuNPs) were repeatedly administered intravenously at three dose levels (0.9, 9 and 90 µg) to male Sprague Dawley rats weekly for 7 weeks, followed by a 14-day washout ... -
The Deep Genome Project
Lloyd, K.C. Kent; Grobler, Anne; Adams, David J.; Baynam, Gareth; Beaudet, Arthur L. (BMC, 2020)In vivo research is critical to the functional dissection of multi-organ systems and whole organism physiology, and the laboratory mouse remains a quintessential animal model for studying mammalian, especially human, ... -
Development and evaluation of a rapid multiplex-PCR based system for Mycobacterium tuberculosis diagnosis using sputum samples
Mutingwende, Isaac; Vermeulen, Urban; Steyn, Faans; Grobler, Anne; Viljoen, Hendrik (Elsevier, 2015)Global tuberculosis (TB) control and eradication is hampered by the unavailability of simple, rapid and affordable diagnostic tests deployable at low infrastructure microscopy centers. We have developed and evaluated the ... -
Development and validation of an LC-MS/MS method for the quantification of goserelin in a Pheroid (R) formulation, in simulated intestinal fluid
Takyi-Williams, John; Erasmus, Linné; Hayeshi, Rose; Grobler, Anne (Elsevier, 2020)The purpose of this reported study was to develop and validate an LC-MS/MS method for the quantification of goserelin in a Pheroid (R) formulation simulated intestinal fluid. Biopharmaceuticals are formulated in drug ... -
Dual radiolabeling as a technique to track nanocarriers: the case of gold nanoparticles
Rambanapasi, Clinton; Barnard, Nicola; Grobler, Anne; Buntting, Hilton; Jordaan, Anine; Steyn, Hendrik; Zeevaart, Jan Rijn (MDPI, 2015)Gold nanoparticles (AuNPs) have shown great potential for use in nanomedicine and nanotechnologies due to their ease of synthesis and functionalization. However, their apparent biocompatibility and biodistribution is still ... -
The effect of the Pheroid delivery system on the in vitro metabolism and in vivo pharmacokinetics of artemisone
Grobler, Lizette; Grobler, Anne; Haynes, Richard; Steyn, Hendrik Stefanus; Masimirembwa, Collen; Thelingwani, Roslyn (Taylor & Francis, 2014)Objectives: The objectives were to determine the pharmacokinetics (PK) of artemisone and artemisone formulated in the Pheroid® drug delivery system in primates and to establish whether the formulation affects the in vitro ... -
Evaluation of a flexible NOTA-RGD kit solution using Gallium-68 from different 68Ge/ 68Ga-generators: pharmacokinetics and biodistribution in nonhuman primates and demonstration of solitary pulmonary nodule imaging in humans
Ebenhan, Thomas; Schoeman, Isabel; Grobler, Anne; Zeevaart, Jan Rijn; Rossouw, Daniel D. (Springer, 2016)Purpose: Radiopharmaceuticals containing the motive tripeptide arginyl-glycyl-asparatic acid (RGD) are known to target ανβ3 integrins during tumor angiogenesis. A more generic kit radiolabeling procedure accommodating ... -
The fabrication and characterization of a PLGA nanoparticle–Pheroid® combined drug delivery system
Chelopo, Madichaba P.; Grobler, Anne; Hayeshi, Rose; Kalombo, Lonji; Wesley-Smith, James (Springer, 2017)The combination of polymeric nanoparticles (NPs) as a core and lipid vesicles as a shell has emerged to be a robust and promising drug delivery strategy. This study explores the development of a novel combined delivery ... -
A model of isoniazid treatment of tuberculosis
Lemmer, Yolandy; Grobler, Anne; Viljoen, Hendrik; Moody, Clint (Elsevier, 2014)A mathematical model is presented of the growth and death of bacilli in a granuloma. The granuloma is treated with isoniazid (INH), a drug that inhibits the synthesis of mycolic acids (MA). Since MA is an essential component ... -
Novel S-adenosyl-L-methionine decarboxylase inhibitors as potent antiproliferative agents against intraerythrocytic Plasmodium falciparum parasites
Le Roux, Dina; Grobler, Anne; Burger, Pieter B.; Niemand, Jandeli; Urbán, Patricia (Elsevier, 2014)S-adenosyl-L-methionine decarboxylase (AdoMetDC) in the polyamine biosynthesis pathway has been identified as a suitable drug target in Plasmodium falciparum parasites, which causes the most lethal form of malaria. ... -
Production of bio-degradable polyurethane foam from sugarcane bagasse and biodiesel-derived crude glycerine
Marx, Sanette; Odendaal, Wilmar; Williams, Anné; Vermeulen, Urban; Grobler, Anne; Muller, L.C. (ETA-Florence Renewable Energies, 2015)As the threat of the depletion of fossil based fuels becomes more of a reality, there is a need to find alternative carbon sources for the production of a wide range of chemicals, polymers and plastics. One plastic that ... -
Pulmonary delivery of anti-tubercular drugs using ligand anchored pH sensitive liposomes for the treatment of pulmonary tuberculosis
Bhardwaj, Ankur; Grobler, Anne; Rath, Goutam; Kumar Goyal, Amit; Mehta, Abhinav (Bentham Science, 2016)Background: Mycobacterium tuberculosis (M. TB) remains the prime cause of bacterial mortality and morbidity world-wide. Therefore, effective delivery and targeting of drug to the cellular tropics is essentially required ... -
Pulmonary delivery of antitubercular drugs using spray-dried lipid-polymer hybrid nanoparticles
Bhardwaj, Ankur; Grobler, Anne; Mehta, Abhinav; Mehta, Shuchi; Yadav, Shailendra (Taylor & Francis, 2016)The present study aimed to develop lipid–polymer hybrid nanoparticles (LPNs) for the combined pulmonary delivery of isoniazid (INH) and ciprofloxacin hydrochloride (CIP HCl). Drug-loaded LPNs were prepared by the ... -
Rapid cell lysis and DNA capture in a lysis microreactor
Grobler, Anne; Levanets, Oksana; Whitney, Scott; Booth, Christine; Viljoen, Hendrik (Elsevier, 2012)In clinical diagnostics, the detection and identification of bacterial organisms based on the analysis of their nucleic acids require cell lysis and DNA extraction from clinical samples. A lysis microreactor (LMR) has been ... -
Role of novel drug delivery systems in bioavailability enhancement: at a glance
Abhinav, Mehta; Grobler, Anne; Neha, Jain; Bharti, Vandana (DYR Labs, 2016)Novel drug delivery systems (NDDS) are one of the most strategies which enable to overcome the problems related to drug bioavailability. It is the rate and extent to which a drug becomes available to the target tissue after ... -
Safety pharmacology in and for Africa: establishment of the pre-clinical drug development platform
Guth, Brian D.; Grobler, Anne (Elsevier, 2018)Southern Africa (South Africa as well as neighboring countries) has not had an infrastructure for testing the safety of new drugs or other therapeutic entities, including plant-based or herbal treatments coming from the ... -
Samarium oxide as a radiotracer to evaluate the in vivo biodistribution of PLGA nanoparticles
Mandiwana, Vusani; Grobler, Anne; Zeevaart, Jan Rijn; Kalombo, Lonji; Venter, Kobus (Springer, 2015)Developing nanoparticulate delivery systems that will allow easy movement and localization of a drug to the target tissue and provide more controlled release of the drug in vivo is a challenge in nanomedicine. The aim of ... -
Synthesis and evaluation of hybrid drugs for a potential HIV/AIDS-malaria combination therapy
Aminake, Makoah N.; Grobler, Anne; Kumar, Vipan; Hans, Renate; Wiesner, Lubbe (Elsevier, 2012)Malaria and HIV are among the most important global health problems of our time and together are responsible for approximately 3 million deaths annually. These two diseases overlap in many regions of the world including ...