Browsing Faculty of Natural and Agricultural Sciences by Author "d3ada9c0-2173-43dc-8a10-e8dc41a84191"
Now showing items 1-6 of 6
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Cell-free DNA: preanalytical variables
Bronkhorst, Abel Jacobus; Aucamp, Janine; Pretorius, Piet J. (Elsevier, 2015)Since the discovery of cell-free DNA (cfDNA) in human blood, most studies have focused on diagnostic and prognostic uses of these markers for solid tumors. Except for some prenatal tests and BEAMing, cfDNA analysis has not ... -
A historical and evolutionary perspective on circulating nucleic acids and extracellular vesicles: circulating nucleic acids as homeostatic genetic entities
Aucamp, Janine; Bronkhorst, Abel Jacobus; Pretorius, Piet J. (Springer, 2016)The quantitative and qualitative differences of circulating nucleic acids (cirNAs) between healthy and diseased individuals have motivated researchers to utilize these differences in the diagnosis and prognosis of various ... -
Kinetic analysis, size profiling, and bioenergetic association of DNA released by selected cell lines in vitro
Aucamp, Janine; Bronkhorst, Abel J.; Peters, Dimetrie L.; Van Dyk, Hayley C.; Van der Westhuizen, Francois H.; Pretorius, Piet J. (Springer, 2017)Although circulating DNA (cirDNA) analysis shows great promise as a screening tool for a wide range of pathologies, numerous stumbling blocks hinder the rapid translation of research to clinical practice. This is related ... -
Methodological variables in the analysis of cell-free DNA
Bronkhorst, Abel Jacobus; Aucamp, Janine; Pretorius, Piet J. (Springer, 2016)In recent years, cell-free DNA (cfDNA) analysis has received increasing amounts of attention as a potential non-invasive screening tool for the early detection of genetic aberrations and a wide variety of diseases, especially ... -
A quantitative assessment of cell-free DNA utilizing several housekeeping genes: measurements from four different cell lines
Aucamp, Janine; Bronkhorst, Abel Jacobus; Wentzel, Johannes F.; Pretorius, Piet J. (Springer, 2016)Quantitative real-time PCR (qPCR) is regularly used to quantify cell-free nucleic acids (cfNAs) in order to identify biomarkers for various pathologies. However, studies have shown notable housekeeping gene expression ... -
Valproic acid alters the content and function of the cell-free DNA released by hepatocellular carcinoma (HepG2) cells in vitro
Aucamp, Janine; Van Dyk, Hayley C.; Bronkhorst, Abel J.; Pretorius, Piet J. (Elsevier, 2017)Background It has long been believed that cell-free DNA (cfDNA) actively released into circulation can serve as intercellular messengers, and their involvement in processes such as the bystander effect strongly support ...