Browsing Faculty of Natural and Agricultural Sciences by Author "Loots, Du Toit"
Now showing items 1-20 of 26
-
Altered fatty acid metabolism due to rifampicin-resistance conferring mutations in the rpoB gene of mycobacterium tuberculosis: mapping the potential of pharmaco-metabolomics for global health and personalized medicine
Du Preez, Ilse; Loots, Du Toit (Mary Ann Liebert Inc, 2012)We investigated the use of gas chromatography mass spectrometry (GC-MS) metabolomics to better characterize rifampicin-resistance by comparing the fatty acid metabolomes of two rpoB mutant Mycobacterium tuberculosis strains ... -
The altered human serum metabolome induced by a marathon
Stander, Zinandré; Luies, Laneke; Mienie, Lodewyk J.; Howatson, Glyn; Loots, Du Toit (Springer, 2018)Introduction Endurance races have been associated with a substantial amount of adverse effects which could lead to chronic disease and long-term performance impairment. However, little is known about the holistic metabolic ... -
An altered mycobacterium tuberculosis metabolome induced by katG mutations resulting in isoniazid resistance
Loots, Du Toit (American Society for Microbiology, 2014)The most common form of drug resistance found in tuberculosis (TB)-positive clinical samples is monoresistance to isoniazid. Various genomics and proteomics studies to date have investigated this phenomenon; however, the ... -
Can metabolomics improve tuberculosis diagnostics?
Du Preez, Ilse; Loots, Du Toit (Springer, 2014)With one third of the global human population suffering from tuberculosis (TB) in 2001, the disease was officially declared a worldwide emergency by the World Health Organization. Since then this disease has grown ... -
A comparison of four sputum pre-extraction preparation methods for identifying and characterising Mycobacterium tuberculosis using GCxGC-TOFMS metabolomics
Schoeman, Johannes C.; Du Preez, Ilse; Loots, Du Toit (Elsevier, 2012)In many pulmonary diseases, sputum is a valuable sample material for use in disease characterisation and diagnostics. However, due to its high viscosity and uneven consistency (lumpiness), it is difficult to obtain ... -
A comparison of two extraction methods for differentiating and characterising various Mycobacterium species and Pseudomonas aeruginosa using GC-MS metabolomics
Olivier, Ilse; Loots, Du Toit (Academic Journals, 2012)We investigated the capacity of a metabolomics research approach to characterise and differentiate between various infectious Mycobacterium species and Pseudomonas aeruginosa, and compared two extraction procedures; 1) ... -
Effects of dieatry onion (Allium cepa L.) in a rodent model of high-fat diet streptozotocin-induced diabetes
Islam, M.S.; Choi, H.; Loots, Du Toit (Karger, 2008)Background/Aims: The present study was conducted to investigate the effects of two dietary doses of freeze-dried onion powder on diabetes-related symptoms in a high-fat (HF) diet streptozotocin (STZ)-induced diabetes rat ... -
Experimental rodent models of type 2 diabetes: a review
Islam, Md. Shahidul; Loots, Du Toit (Thomson Reuters, 2009)Due to the high prevalence of diabetes worldwide, extensive research is still being performed to develop new antidiabetic agents and determine their mechanisms of action. Consequently, a number of diabetic animal models ... -
Food antioxidant capacity and its use in food selection
Loots, Du Toit (MedPharm Publications, 2009)In the current issue of the Journal, Louwrens and co-workers determine the per capita South African daily dietary total antioxidant capacity (TAC)1 using published data by Nel and Steyn (2002) which summarises the food ... -
Glycaemic control improves fibrin network characteristics in type 2 diabetes: a purified fibrinogen model
Pieters, Marlien; Van der Westhuizen, Francois H.; Loots, Du Toit; Jerling, Johann C.; Elgar, Dale (Thieme, 2008)Diabetic subjects have been shown to have altered fibrin network structures.One proposed mechanism for this is non-enzymatic glycation of fibrinogen due to high blood glucose.We investigated whether glycaemic control would ... -
Ileal and faecal digestibility of daidzein and genistein and plasma bioavailability of these isoflavones and their bioactive metabolites in the ovariectomised rat
Poulsen, Raewyn C.; Loots, Du Toit; Moughan, Paul J.; Kruger, Marlena C. (Wiley, 2009)Consumption of the soya isoflavones genistein and daidzein may provide protection against postmenopausal bone loss. The purpose of this study was to determine ileal and faecal digestibility of daidzein and genistein and ... -
In vitro antioxidant, antimutagenic and genoprotective activity of Rosa roxburghii fruit extract
Van der Westhuizen, Francois H.; Janse van Rensburg, Catharina S.; Rautenbach, George S.; Loots, Du Toit; Huysamen, Cristal; Louw, Roan; Pretorius, Pieter J.; Erasmus, Elardus; Marnewick, Jeanine L. (Wiley, 2008)The antioxidant properties of the fruit of the Rosa roxburghii (RR) plant have been associated with several putative health promoting effects. The possible cytotoxic, mutagenic/antimutagenic and genotoxic effects of RR ... -
Linolenic acid and folate in wild-growing African dark leafy vegetables (morogo)
Van der Walt, Anna M.; Ibrahim, Mohamed I.M.; Bezuidenhout, Cornelius C.; Loots, Du Toit (Cambridge Univ Press, 2008)Transition from a low-fat vegetable-rich rural diet to a high-fat Westernised diet is considered a factor in the escalating occurrence of vascular-related diseases and type 2 diabetes in urban black South Africans. Consumption ... -
Metabolic characterization of tuberculous meningitis in a South African paediatric population using 1H NMR metabolomics
Van Zyl, Christiaan De Wet; Loots, Du Toit; Van Reenen, Mari; Mason, Shayne; Solomons, Regan (Elsevier, 2020)Objective To better characterize the cerebrospinal fluid (CSF) metabolic profile of tuberculous meningitis (TBM) cases using a South African paediatric cohort. Methods 1H NMR metabolomics was used to analyse the CSF ... -
A metabolomics approach exploring the function of the ESX-3 type VII secretion system of M. smegmatis
Loots, Du Toit; Meissner-Roloff, Reinart J.; Newton-Foot, Mae; Gey van Pittius, Nicolaas C. (Springer, 2013)The genome of Mycobacterium, including Mycobacterium tuberculosis, contains five copies of a cluster of genes encoding a novel type VII secretion system, named the ESX gene cluster region. This ESX-3 gene cluster is ... -
A metabolomics approach to characterise and identify various Mycobacterium species
Olivier, Ilse; Loots, Du Toit (Elsevier, 2012)We investigated the potential use of gas chromatography mass spectrometry (GC–MS), in combination with multivariate statistical data processing, to build a model for the classification of various tuberculosis (TB) causing, ... -
Metabolomics describes previously unknown toxicity mechanisms of isoniazid and rifampicin
Combrink, Monique; Loots, Du Toit; Du Preez, Ilse (Elsevier, 2020)Isoniazid and rifampicin are well-known anti-mycobacterial agents and are widely used to treat pulmonary tuberculosis (TB) as part of the combined therapy approach, recommended by the World Health Organization. The ingestion ... -
A metabolomics investigation of a hyper- and hypo-virulent phenotype of Beijing lineage M. tuberculosis
Meissner-Roloff, Reinart J.; Koekemoer, Gerhard; Loots, Du Toit; Warren, Robert M. (Springer, 2012)Despite a number investigations using rapid sequencing and comparative genomic techniques, attempting to characterise the phenomenon of varying degrees of virulence within the Mycobacterium tuberculosis species, the ... -
Overview of brain-to-gut axis exposed to chronic CNS bacterial infection(s) and a predictive urinary metabolic profile of a brain infected by mycobacterium tuberculosis
Isaiah, Simon; Loots, Du Toit; Mason, Shayne; Solomons, Regan; Van der Kuip, Martijn (Frontiers Media, 2020)A new paradigm in neuroscience has recently emerged – the brain–gut axis (BGA). The contemporary focus in this paradigm has been gut → brain (“bottom-up”), in which the gut-microbiome, and its perturbations, affects one’s ... -
Phytochemical contents and antioxidants cappacities of two Aloe greatheadii var. davyana extracts
Botes, Lisa; Van der Westhuizen, Francois; Loots, Du Toit (MDPI, 2008)Aloe greatheadii var. davyana (Asphodelaceae) is used among rural South African communities to treat arthritis, skin cancer, burns, eczema, psoriasis, digestive problems, high blood pressure and diabetes, despite very ...