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dc.contributor.authorBronkhorst, Abel Jacobus
dc.contributor.authorAucamp, Janine
dc.contributor.authorPretorius, Piet J.
dc.date.accessioned2017-04-18T11:39:05Z
dc.date.available2017-04-18T11:39:05Z
dc.date.issued2016
dc.identifier.citationBronkhorst, A.J. et al. 2016. Methodological variables in the analysis of cell-free DNA. (In Gahan, P.B., Fleischhacker, M & Schmidt, B., eds. Circulating nucleic acids in serum and plasma – CNAPS IX). Advances in experimental medicine and biology, 924:157-163. [https://doi.org/10.1007/978-3-319-42044-8_29]en_US
dc.identifier.isbn978-3-319-42044-8
dc.identifier.urihttp://hdl.handle.net/10394/21441
dc.identifier.urihttps://doi.org/10.1007/978-3-319-42044-8_29
dc.identifier.urihttps://link.springer.com/chapter/10.1007%2F978-3-319-42044-8_29
dc.description.abstractIn recent years, cell-free DNA (cfDNA) analysis has received increasing amounts of attention as a potential non-invasive screening tool for the early detection of genetic aberrations and a wide variety of diseases, especially cancer. However, except for some prenatal tests and BEAMing, a technique used to detect mutations in various genes of cancer patients, cfDNA analysis is not yet routinely applied in clinical practice. Although some confusing biological factors inherent to the in vivo setting play a key part, it is becoming increasingly clear that this struggle is mainly due to the lack of an analytical consensus, especially as regards quantitative analyses of cfDNA. In order to use quantitative analysis of cfDNA with confidence, process optimization and standardization are crucial. In this work we aim to elucidate the most confounding variables of each preanalytical step that must be considered for process optimization and equivalence of proceduresen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.subjectCell-free DNAen_US
dc.subjectCanceren_US
dc.subjectPreanalytical factorsen_US
dc.subjectPrenatal diagnosticsen_US
dc.subjectQuantitative analysisen_US
dc.titleMethodological variables in the analysis of cell-free DNAen_US
dc.typeBook chapteren_US
dc.contributor.researchID22195289 - Bronkhorst, Abel Jacobus
dc.contributor.researchID20505698 - Aucamp, Janine
dc.contributor.researchID10176705 - Pretorius, Petrus Jacobus


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