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dc.contributor.authorJudefeind, Anja
dc.contributor.authorJansen van Rensburg, Peet
dc.contributor.authorDu Plessis, Jeanetta
dc.contributor.authorLangelaar, Stephan
dc.contributor.authorWiechers, Johann W.
dc.date.accessioned2009-08-25T09:11:05Z
dc.date.available2009-08-25T09:11:05Z
dc.date.issued2008
dc.identifier.citationJudefeind, A. et al. 2008. Quantitative determination of octadecenedioic acid in human skin and transdermal perfusates by gas chromatography-mass spectrometry. Journal of chromatographic science, 46(6):544-550. [https://doi.org/10.1093/chromsci/46.6.544]en
dc.identifier.issn0021-9665
dc.identifier.urihttp://hdl.handle.net/10394/2161
dc.identifier.urihttps://doi.org/10.1093/chromsci/46.6.544
dc.identifier.urihttps://academic.oup.com/chromsci/article/46/6/544/314800/Quantitative-Determination-of-Octadecenedioic-Acid
dc.description.abstractA gas chromatographic (GC) method with mass spectrometric (MS) detection is developed and validated for the accurate and precise determination of octadecenedioic acid (C18:1 DIOIC) in human skin samples and transdermal perfusates. C18:1 DIOIC is extracted using methanol. The saturated analogue 1,18-octadecanedioic acid (C18:0 DIOIC) is added as internal standard. Prior to analysis, both compounds are converted to their trimethylsilylated derivatives using N,O-bis(trimethylsilyl)trifluoroacetamide with 15% trimethylchlorosilane. Quantitation is performed in selected ion monitoring mode with a limit of quantitation of 250 ng/mL. Linearity with a correlation coefficient of 0.998 is obtained over a concentration range of 250-2000 ng/mL. Values for within-day accuracy range from 94.5% to 102.4%, and from 97.5% to 105.8% for between-day accuracy. Within- and between-day precision values are better than 5% and 7%, respectively. The recovery values from the various matrices vary from 92.6% to 104.0%. The GC-MS method is employed for the determination of C18:1 DIOIC after application of an emulsion containing the active ingredient onto human skin in vitro. The results demonstrate that the method is suitable for the determination of C18:1 DIOIC in human skin samples and transdermal perfusates
dc.language.isoenen
dc.publisherOxford Univ Press
dc.titleQuantitative determination of octadecenedioic acid in human skin and transdermal perfusates by gas chromatography-mass spectrometry.en
dc.typeArticleen
dc.contributor.researchID10211705 - Jansen van Rensburg, Petrus Johannes
dc.contributor.researchID10065318 - Du Plessis, Jeanetta
dc.contributor.researchID12878332 - Otto, Anja


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