Show simple item record

dc.contributor.authorBrink, Christiaan B.
dc.contributor.authorPretorius, Anita
dc.contributor.authorVan Niekerk, Barend P.J.
dc.contributor.authorOliver, Douglas W.
dc.contributor.authorVenter, Daniel P.
dc.date.accessioned2010-01-08T12:09:17Z
dc.date.available2010-01-08T12:09:17Z
dc.date.issued2008
dc.identifier.citationBrink, C.B. et al. 2008. Studies on cellular resilience and adaptation following acute and repetitive exposure to ozone in cultured human epithelial (HeLa) cells. Redox report, 13(2):87-100. [https://doi.org/10.1179/135100008X259187]en
dc.identifier.issn1351-0002
dc.identifier.issn1743-2928 (Online)
dc.identifier.urihttp://hdl.handle.net/10394/2736
dc.identifier.urihttps://www.tandfonline.com/doi/abs/10.1179/135100008X259187
dc.identifier.urihttps://doi.org/10.1179/135100008X259187
dc.description.abstractOzone is used to treat several medical conditions, while the underlying mechanisms of action are sometimes poorly understood. In the current study, we exposed cultured human epithelial (HeLa) cells acutely and repeatedly to ozone and investigated the effects thereof on cell viability. The involvement of anti-apoptotic pathways in observed adaptive responses to ozone were investigated by employing the Akt inhibitor (–)-deguelin. Cells were exposed to an ozone-saturated physiological solution using various dosing regimens, including acute exposure and various repetitive exposures. Cell viability was determined with Trypan Blue or MTT tests, or by a DNA-fragmentation (comet) assay. Acute ozone exposure compromised cell membrane integrity severely, while adaptation to reverse an initial reduction in mitochondrial activity was observed. Repetitive, short-duration exposures followed by a single long-duration exposure to ozone furnished a protective adaptation that was reversed by Akt inhibition. Extracellular and intracellular damage (and adaptation) occurs differentially. While acute ozone may decrease cell viability, multiple preexposures up-regulates cellular plasticity via induction of anti-apoptotic pathways in a treatment regimen-specific manner
dc.language.isoenen
dc.publisherTaylor & Francisen
dc.subjectOzone
dc.subjectAdaptation
dc.subjectMembrane integrity
dc.subjectMitochondrial activity
dc.subjectDNA integrity
dc.subjectHuman epithelial cells
dc.titleStudies on cellular resilience and adaptation following acute and repetitive exposure to ozone in cultured human epithelial (HeLa) cellsen
dc.typeArticleen
dc.contributor.researchID10073892 - Brink, Christiaan Beyers
dc.contributor.researchID10060855 - Oliver, Douglas William


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record