dc.contributor.author | Ndaba, Busiswa | |
dc.contributor.author | Chiyanzu, Idan | |
dc.contributor.author | Marx, Sanette | |
dc.date.accessioned | 2020-09-01T08:03:20Z | |
dc.date.available | 2020-09-01T08:03:20Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Ndaba, B. et al. 2015. Bio-butanol fermentation using clostridium acetobutylicum and clostridium tetanomorphum in modified bioreactor flasks. Proceedings 23rd European Biomass Conference and Exhibition, 1-4 June, Vienna, Austria: 351-355. [https://doi.org/10.5071/23rdEUBCE2015-1CV.3.53] | en_US |
dc.identifier.isbn | 978-88-89407-516 | |
dc.identifier.issn | 2282-5819 | |
dc.identifier.uri | http://hdl.handle.net/10394/35669 | |
dc.identifier.uri | http://www.etaflorence.it/proceedings/index.asp?detail=11243 | |
dc.identifier.uri | https://doi.org/10.5071/23rdEUBCE2015-1CV.3.53 | |
dc.description.abstract | An alternative new generation biofuel to bio-ethanol has attracted researchers to investigate various approaches for optimization of traditional ABE fermentation to enable bio-butanol to be produced cheaper at an industrial scale. For instance, the use of different or modified Clostridium strains to enhance bio-butanol yields has been extensively studied. In this study, single-culture fermentation was investigated usingdifferentinoculum concentrations of Clostridium acetobutylicum andClostridiumtetanomorphum(3, 5, 10 % v/v) to ferment sweet sorghum juicewith a Brix index of 14°.Furthermore, co-culture fermentation were conducted using varying ratios of the two clostridium speciesin modified reactor flasks at pH 6-6.5, and incubated at 37oC while shaking (150rpm) for 96 hrs. Cell growth profileswere monitored using UV spectrophotometry at 600nm during both single and co-culture fermentation. Meanwhile, bio-butanol, residual sugars and by-products were analysed by high-performance liquid chromatography (HPLC).The results demonstrated that butanol and organic acids can be produced from sweet sorghum juice using a single culture, whereas co-culture only produced acids with no organic solvents. The highest butanol concentrationof6.49 g/L was obtained after96hrs using 10 % v/v C. acetobutylicumas a single culture. The highest organic acid concentration (Lactic acid) of 2.7 g/L was produced when an inoculum ratio of 6.5: 6.5 %v/v C. acetobutylicum to C. tetanomorphum were used and fermented for 96 hrs. Sweet sorghum juicehas potential as a cheaper substrate in producing high bio-butanol yield as a product in the presence of C.acetobutylicum. Co-culturescan also be used in fermentation of interest to produce high yields of organic acids | en_US |
dc.language.iso | en | en_US |
dc.publisher | ETA-Florence Renewable Energies | en_US |
dc.subject | Fermentation | en_US |
dc.subject | Bio-butanol | en_US |
dc.subject | Clostridium species | en_US |
dc.subject | Sweet sorghum juice | en_US |
dc.subject | Organic acids | en_US |
dc.title | Bio-butanol fermentation using clostridium acetobutylicum and clostridium tetanomorphum in modified bioreactor flasks | en_US |
dc.type | Presentation | en_US |
dc.contributor.researchID | 10216847 - Marx, Sanette | |
dc.contributor.researchID | 24043605 - Chiyanzu, Idan | |
dc.contributor.researchID | 23104414 - Ndaba, Busiswa | |