Die effek van sikliese adenosien-3':5'-monofosfaat op hemoglobien-biosintese
Abstract
Although cAMP may be involved in the regulation of protein biosynthesis in certain eukaryotic cells no decisive relationship
between hormonal action, cAMP and the regulation of translation
could be found in the literature. Since the evidence of an effect of cAMP on the translation of mRNA was not unequivocally
established, the object of this study was to investigate the
effect of cAMP on the synthesis of globin in rabbit reticulocyte
in situ and in vitro systems.
A stimulation of polypeptide synthesis, i.e. globin biosynthesis,
by cAMP could be shown in both systems. In case of the in vitro
system the optimal cAMP concentration varied between 4 and 24
mM, whereas the stimulatory effect amounted between 30 and
40C/% . This investigation was fu rther extended to determine the
effects of dB cAMP, aminophyline and 5' -AMP on polypeptide synthesis.
With regard to the mechanism through which cAMP induce the observed stimulation of protein biosynthesis, an inverse relationship between the presence of an energy regeneration system and
ATP and the stimulatory effect of cAMP was found. It was further
shown that cAMP stimulates all the translation phases.
Possible explanations of the observed results are given.