Pheroid technology for the topical delivery of depigmenting agents transforming growth factor–ß1 and tumor necrosis factor–a / Berenice Campbell

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dc.contributor.author Campbell, Berenice en_US
dc.date.accessioned 2011-09-08T07:00:09Z
dc.date.available 2011-09-08T07:00:09Z
dc.date.issued 2010 en_US
dc.identifier.uri http://hdl.handle.net/10394/4739
dc.description Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2011.
dc.description.abstract Pigmentation disorders occur in multiple conditions (Hakozaki et al., 2006:105). Although many modalities of treatments are available, none are completely satisfactory (Briganti et al., 2003:101). Two cytokines normally present in the skin, transforming growth factor–beta1 (TGF–81) and tumour necrosis factor–alpha (TNF–9), have been shown to inhibit melanin synthesis (Martinez–Esparza, 2001:972). The stratum corneum has been commonly accepted as the main barrier to percutaneous absorption. Many techniques have been applied to overcome this barrier properties and to enhance penetration with varying success (Pellet et al., 1997:92). The objective of this study was to investigate the topical delivery of the above mentioned peptide drugs with aid of the Pheroid drug delivery system. Pheroid technology is a delivery system that promotes the absorption and increases the efficacy of dermatological, biological and oral medicines in various pharmacological groups (Grobler et al., 2008:4). Pheroid entraps drugs with high efficiency and delivers them with remarkable speed to target sites (Grobler, 2004:4). In order to avoid degradation of these peptides, bestatin hydrochloride (an aminopeptidase inhibitor), was used (Lkhagvaa et al., 2008:386). Topical drug delivery was achieved by means of vertical Franz cell diffusion studies performed over a 6 and 12 h period. ELISA (enzyme linked immunosorbent assay) detection was used to detect cytokine concentrations. Entrapped cytokine solutions were monitored by confocal laser scanning microscopy (CLSM). Upon removal of donor and receptor compartments, skin discs were subjected to tape stripping in order to establish the amount of active present within the stratum corneum and epidermis as well as the remaining dermis (Pellet et al., 1997:92). When comparing the two studies with each other, it is evident that the diffused concentration values obtained with PBS (phosphate buffer solution, pH 7.4) was lower than that obtained with the Pheroid drug delivery system. Both cytokine concentrations were successfully delivered topically as a minimum of concentrations for both actives were detected. This positive result was confirmed as well by the amount of active detected in stratum corneum–epidermis and epidermis–dermis solutions. en_US
dc.publisher North-West University
dc.subject Pigmentation en_US
dc.subject Topical delivery en_US
dc.subject Pheroid en_US
dc.subject Transforming growth factor-beta1 en_US
dc.subject Tumour necrosis factor-alpha en_US
dc.subject Bestatin en_US
dc.subject Tape stripping en_US
dc.subject Pigmentasie en_US
dc.subject Topikale aflewering en_US
dc.subject Transformerende groei faktor-beta1 en_US
dc.subject Tumor nekrosis faktor-alpha en_US
dc.subject Bestatien en_US
dc.subject Bandstroping en_US
dc.title Pheroid technology for the topical delivery of depigmenting agents transforming growth factor–ß1 and tumor necrosis factor–a / Berenice Campbell en_US
dc.type Thesis en_US
dc.description.thesistype Masters en_US

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