Optimization of the enzymatic conversion of maize stover to bioethanol / by Nombongo Mabentsela

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dc.contributor.author Mabentsela, Nombongo en_US
dc.date.accessioned 2011-10-03T08:45:18Z
dc.date.available 2011-10-03T08:45:18Z
dc.date.issued 2010 en_US
dc.identifier.uri http://hdl.handle.net/10394/4852
dc.description Thesis (M.Sc. Engineering Sciences (Chemical Engineering))--North-West University, Potchefstroom Campus, 2011.
dc.description.abstract The severe effects associated with global warming and the rapid increase in oil prices are the driving forces behind the demand for clean carbon–neutral and biofuels such as bioethanol. Research studies are now focusing on using lignocellulosic biomass for bioethanol production due to concerns about food security and inflation. The chosen feedstock for this study was maize stover, given that it is the most abundant agricultural residue in South Africa. Maize stover consists of structural carbohydrates that can be enzymatically converted into fermentable sugars. The major drawback in the production of bioethanol from lignocellulosic biomass has been its high equipment and operational costs due to the use of acids and high enzyme loadings. The aim of this study was to investigate the possibility of optimizing the enzyme hydrolysis of pre–treated maize stover without further increasing the amount of enzymes. The maximum glucose yield attained was 690 ± 35 mg of glucose per gram of substrate which is equivalent to a conversion efficiency of 119%. The preferred pre–treatment method used was 3% sulphuric acid for 60 minutes at 121oC and the enzymatic hydrolysis process was performed at a 5% substrate loading, 50oC and pH 5.0 using 30 FPU per gram of cellulose in the presence of 1.25 g.L–1 of Tween 80 for 48 hours. The addition of Tween 80 increased the glucose yields by 23 % and thus, it has the potential of lowering the overall process costs by increasing the glucose yield without further addition of enzymes. Keywords: Bioethanol, maize stover, lignocellulosic biomass, pre–treatment, enzymatic hydrolysis en_US
dc.publisher North-West University
dc.subject Bioethanol en_US
dc.subject Maize stover en_US
dc.subject Lignocellulosic biomass en_US
dc.subject Pre-treatment en_US
dc.subject Enzymatic hydrolysis en_US
dc.subject Tween 80 en_US
dc.subject Bioetanol en_US
dc.subject Mieliestronke en_US
dc.subject Houtagtige sellulose-biomassa en_US
dc.subject Voorbehandeling en_US
dc.subject Ensiemhidrolise en_US
dc.title Optimization of the enzymatic conversion of maize stover to bioethanol / by Nombongo Mabentsela en_US
dc.type Thesis en_US
dc.description.thesistype Masters en_US

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    This collection contains the original digitized versions of research conducted at the North-West University (Potchefstroom Campus)

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