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dc.contributor.authorWentzel, Johannes F.en_US
dc.contributor.authorGouws, Chrisnaen_US
dc.contributor.authorHuysamen, Cristalen_US
dc.contributor.authorVan Dyk, Etresiaen_US
dc.contributor.authorKoekemoer, Gerharden_US
dc.contributor.authorPretorius, Pieter J.en_US
dc.identifier.citationWentzel, J.F. et al. 2010. Assessing the DNA methylation status of single cells with the comet assay. Analytical biochemistry, 400(2):190-194. []en_US
dc.description.abstractThe comet assay (single cell gel electrophoresis) is a cost-effective, sensitive, and simple technique that is traditionally used for analyzing and quantifying DNA damage in individual cells. The aim of this study was to determine whether the comet assay could be modified to detect changes in the levels of DNA methylation in single cells. We used the difference in methylation sensitivity of the isoschizomeric restriction endonucleases HpaII and MspI to demonstrate the feasibility of the comet assay to measure the global DNA methylation level of individual cells. The results were verified with the well-established cytosine extension assay. We were able to show variations in DNA methylation after treatment of cultured cells with 5-azacytidine and succinylacetone, an accumulating metabolite in human tyrosinemia type I
dc.subjectComet assay
dc.subjectDNA methylation
dc.subjectCytosine extension assay
dc.subjectTyrosinemia type I
dc.titleAssessing the DNA methylation status of single cells with the comet assayen_US
dc.contributor.researchID12450960 - Gouws, Chrisna
dc.contributor.researchID10096353 - Koekemoer, Gerhard
dc.contributor.researchID10176705 - Pretorius, Petrus Jacobus
dc.contributor.researchID12126497 - Van Dyk, Etresia
dc.contributor.researchID20134045 - Wentzel, Johannes Frederik

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