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dc.contributor.authorScheepers, Johanna C.
dc.contributor.authorMalan, Sarel F.
dc.contributor.authorDu Preez, Jan L.
dc.contributor.authorVan Dyk, Sandra
dc.date.accessioned2012-08-28T13:37:30Z
dc.date.available2012-08-28T13:37:30Z
dc.date.issued2011
dc.identifier.citationScheepers, J.C. et al. 2011. The high performance liquid chromatography (HPLC) analysis of ultraviolet (UV) irradiated chlorophyll a and secondary plant compounds. African journal of biotechnology, 10(74):16976-16985. [http://dx.doi.org/10.5897/AJB11.1999]en_US
dc.identifier.issn1684-5315
dc.identifier.urihttp://hdl.handle.net/10394/7132
dc.identifier.urihttps://www.ajol.info/index.php/ajb/article/view/97780
dc.identifier.urihttps://www.ajol.info/index.php/ajb/article/view/97780/87082
dc.identifier.urihttp://dx.doi.org/10.5897/AJB11.1999
dc.description.abstractA large number of diseases such as cancer, cardiovascular disease, inflammatory disease, neurodegenerative disorders and ageing are consequences of reactive oxygen species (ROS) and reactive nitrogen species (RNS). Deoxyribonucleic acid (DNA) damage, lipid peroxidation and protein damage are biomarkers for damage in the cell caused by ROS and RNS. Various plant extracts have been tested for anti-oxidant properties. Due to the chlorophyll in the extracts, false negative results from spectrophotometric tests are often observed. A pilot study was done to determine the stability of the secondary compounds with high performance liquid chromatography (HPLC) after the samples were bleached with ultraviolet (UV) radiation and treated with activated charcoal. The chlorophyll a was successfully degraded with UV radiation in all the samples leaving a bleached extract suitable for biological assays.en_US
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.subjectChlorophyll aen_US
dc.subjectUV radiationen_US
dc.subjectactivated charcoalen_US
dc.subjectHPLCen_US
dc.subjectsecondary compounds in plant extractsen_US
dc.titleThe high performance liquid chromatography (HPLC) analysis of ultraviolet (UV) irradiated chlorophyll a and secondary plant compoundsen_US
dc.typeArticleen_US
dc.contributor.researchID10065911 - Van Dyk, Sandra
dc.contributor.researchID10199667 - Malan, Sarel Francois
dc.contributor.researchID10060510 - Du Preez, Jan Lourens
dc.contributor.researchID11311827 - Scheepers, Johanna Cornelia


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