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The high performance liquid chromatography (HPLC) analysis of ultraviolet (UV) irradiated chlorophyll a and secondary plant compounds

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dc.contributor.author Malan, Sarel Francois
dc.contributor.author Scheepers, Johanna Cornelia
dc.contributor.author Van Dyk, Sandra
dc.contributor.author Du Preez, Jan Lourens
dc.date.accessioned 2012-08-28T13:37:30Z
dc.date.available 2012-08-28T13:37:30Z
dc.date.issued 2011
dc.identifier.citation Scheepers, J.C. et al. 2011. The high performance liquid chromatography (HPLC) analysis of ultraviolet (UV) irradiated chlorophyll a and secondary plant compounds. African journal of biotechnology, 10(74):16976-16985. [http://www.academicjournals.org/journal/AJB] en_US
dc.identifier.issn 1684-5315
dc.identifier.uri http://hdl.handle.net/10394/7132
dc.description.abstract A large number of diseases such as cancer, cardiovascular disease, inflammatory disease, neurodegenerative disorders and ageing are consequences of reactive oxygen species (ROS) and reactive nitrogen species (RNS). Deoxyribonucleic acid (DNA) damage, lipid peroxidation and protein damage are biomarkers for damage in the cell caused by ROS and RNS. Various plant extracts have been tested for anti-oxidant properties. Due to the chlorophyll in the extracts, false negative results from spectrophotometric tests are often observed. A pilot study was done to determine the stability of the secondary compounds with high performance liquid chromatography (HPLC) after the samples were bleached with ultraviolet (UV) radiation and treated with activated charcoal. The chlorophyll a was successfully degraded with UV radiation in all the samples leaving a bleached extract suitable for biological assays. en_US
dc.description.uri http://www.academicjournals.org/AJB/PDF/pdf2011/23Nov/Scheepers%20et%20al.pdf
dc.description.uri http://dx.doi.org/10.5897/AJB11.1999
dc.language.iso en en_US
dc.publisher Academic journals en_US
dc.subject Chlorophyll a en_US
dc.subject UV radiation en_US
dc.subject activated charcoal en_US
dc.subject HPLC en_US
dc.subject secondary compounds in plant extracts en_US
dc.title The high performance liquid chromatography (HPLC) analysis of ultraviolet (UV) irradiated chlorophyll a and secondary plant compounds en_US
dc.type Article en_US


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