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Mitochondrial DNA replication and OXPHOS gene transcription show varied responsiveness to Rieske protein knockdown in 143B cells

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dc.contributor.author Van Plessis, Lissinda Hester
dc.contributor.author Levanets, Oskana
dc.contributor.author Louw, Roan
dc.contributor.author Pretorius, Petrus Jacobus
dc.contributor.author Reinecke, Fimmie
dc.contributor.author Nijtmans, Leo
dc.contributor.author Smeitink, Jan A.M.
dc.contributor.author Van der Westhuizen, Francois Hendrikus
dc.date.accessioned 2012-09-10T15:42:05Z
dc.date.available 2012-09-10T15:42:05Z
dc.date.issued 2011
dc.identifier.citation Levanets, O. et al. 2011. Mitochondrial DNA replication and OXPHOS gene transcription show varied responsiveness to Rieske protein knockdown in 143B cells. Biochimie, 93:758-765. [ URL ] en_US
dc.identifier.issn 0300-9084
dc.identifier.issn 1638-6183 (Online)
dc.identifier.uri http://hdl.handle.net/10394/7263
dc.description.abstract Genetic, biochemical and phenotypic diversity is a hallmark of OXPHOS deficiencies. Among the responses frequently reported for these deficiencies is differential expression of several genes involved in mitochondrial biogenesis. These responses are often associated with elevated oxygen radical production. The genetic diversity of tissue and cell lines used in these investigations, however, complicates the interpretation of observations. We investigated mtDNA copy number and selected transcriptional responses after inducing stable Rieske protein knockdown in 143B cells. Despite the significant loss of complex III activity, hydrogen peroxide levels remained comparable to controls. Furthermore, no significant change in mtDNA copy number was observed. Mitochondrial L-strand- and D-loop transcript levels remained unchanged, while the H-strand transcript for COXII was reduced. With the exception of mitochondrial single-stranded binding protein (mtSSB), which was reduced, no transcriptional changes of the mtDNA replication and transcription machinery were observed. Notably however, a selection of nuclear-encoded OXPHOS gene transcripts was generally reduced (statistically insignificant), except for NDUFS3 and COX4I1 transcripts, which were significantly reduced. From these results we conclude that the induction of a low superoxide producing complex III deficiency in 143B cells has an insignificant effect on mtDNA replication and function, but that expression of OXPHOS genes is generally down regulated. This may indicate a lowering of mitochondrial biogenesis and a shift towards anaerobic energy metabolism to improve cellular survival. en_US
dc.description.uri http://dx.doi.org/10.1016/j.biochi.2011.01.004
dc.language.iso en en_US
dc.publisher Editions Scientifique/Medical Elsevier en_US
dc.subject OXPHOS deficiency en_US
dc.subject gene expression en_US
dc.subject mtDNA en_US
dc.subject Rieske knockdown en_US
dc.title Mitochondrial DNA replication and OXPHOS gene transcription show varied responsiveness to Rieske protein knockdown in 143B cells en_US
dc.type Article en_US


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