mirage

Three-dimensional visualisation of developmental stages of an apicomplexan fish blood parasite in its invertebrate host

Boloka/Manakin Repository

Show simple item record

dc.contributor.author Smit, Nicholas Jacobus
dc.contributor.author Hayes, Polly M.
dc.contributor.author Wertheim, David F.
dc.contributor.author Seddon, Alan M.
dc.contributor.author Davies, Angela J.
dc.date.accessioned 2012-11-19T10:27:28Z
dc.date.available 2012-11-19T10:27:28Z
dc.date.issued 2011
dc.identifier.citation Hayes, P.M. et al. 2011. Three-dimensional visualisation of developmental stages of an apicomplexan fish blood parasite in its invertebrate host. Parasites & Vectors, 4(219):1-5. [http://www.parasitesandvectors.com/] en_US
dc.identifier.issn 1756-3305
dc.identifier.issn 1756-3305 (Online)
dc.identifier.uri http://hdl.handle.net/10394/7772
dc.description.abstract Background Although widely used in medicine, the application of three-dimensional (3D) imaging to parasitology appears limited to date. In this study, developmental stages of a marine fish haemogregarine, Haemogregarina curvata (Apicomplexa: Adeleorina), were investigated in their leech vector, Zeylanicobdella arugamensis; this involved 3D visualisation of brightfield and confocal microscopy images of histological sections through infected leech salivary gland cells. Findings 3D assessment demonstrated the morphology of the haemogregarine stages, their spatial layout, and their relationship with enlarged host cells showing reduced cellular content. Haemogregarine meronts, located marginally within leech salivary gland cells, had small tail-like connections to the host cell limiting membrane; this parasite-host cell interface was not visible in two-dimensional (2D) light micrographs and no records of a similar connection in apicomplexan development have been traced. Conclusions This is likely the first account of the use of 3D visualisation to study developmental stages of an apicomplexan parasite in its invertebrate vector. Elucidation of the extent of development of the haemogregarine within the leech salivary cells, together with the unusual connections between meronts and the host cell membrane, illustrates the future potential of 3D visualisation in parasite-vector biology. en_US
dc.description.uri http://dx.doi.org/10.1186/1756-3305-4-219
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.title Three-dimensional visualisation of developmental stages of an apicomplexan fish blood parasite in its invertebrate host en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search the NWU Repository


Advanced Search

Browse

My Account

Statistics