Extraction of lutein from marigold flower with supercritical carbon dioxide
Van Scheltinga, Mariska
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The objective of the study was to extract lutein, an active component of marigold flower (Tagetes erecta) by means of supercritical carbon dioxide (sc-C02). Extractions on freeze-dried marigold petals were performed with a laboratory scale supercritical fluid extractor (LECO TFEIM2000) of highly advanced design and performance. Extracts were analysed for lutein by high-performance liquid chromatography (Agilent Technology 1100 Series HPLC System) using a calibration line constructed by virtue of solutions of a commercially available standard (Indofine Chemical Company, Inc.). The conditions at which a maximum yield of lutein is obtained (52°C, 400 bar, 60 min) were determined by performing runs according to a statistical design and processing the data by computer assisted surface response analysis. A mass balance proved effective recovery of the extracted material from the supercritical extractor. The density of sc-C02 was found to be the variable which controls the extraction as the fluid becomes capable to chemically dissolve lutein once its density (and thus solvent strength) takes on liquid-like values (between 0.8 and 1.0 g/mL). The large negative value of the volume of activation ∆*V relates to the decrease in volume associated with melting and solvation of lutein while it dissolves in the highly compressed supercritical fluid. A function combining several process variables into a dimensionless quantity was used successfully to mathematically describe the extraction process.
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