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dc.contributor.authorMlera, Luwanika
dc.contributor.authorO'Neill, Hester G.
dc.contributor.authorJere, Khuzwayo C.
dc.contributor.authorVan Dijk, Alberdina A.
dc.date.accessioned2015-03-20T09:48:00Z
dc.date.available2015-03-20T09:48:00Z
dc.date.issued2013
dc.identifier.citationMlera, L. et al. 2013. Whole-genome consensus sequence analysis of a South African rotavirus SA11 sample reveals a mixed infection with two close derivatives of the SA11-H96 strain. Archives of virology, 158:1021-1030. [https://doi.org/10.1007/s00705-012-1559-5]en_US
dc.identifier.issn0304-8608
dc.identifier.issn1432-8798 (Online)
dc.identifier.urihttp://hdl.handle.net/10394/13600
dc.identifier.urihttps://doi.org/10.1007/s00705-012-1559-5
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00705-012-1559-5
dc.description.abstractWhole-genome, sequence-independent amplification and 454 pyrosequencing of a rotavirus SA11 cell culture sample with an unknown passage history yielded consensus sequences of twelve complete genome segments. Two distinct sequences for genome segment 8 (encoding NSP2) were present, indicating a mixed infection with two rotavirus SA11 strains. The genotypes of the viruses were G3-P[2]-I2-R2-C5-M5-A5-Nx-T5-E2-H5, where x was either 5 or 2. The strains were named RVA/ Simian-tc/ZAF/SA11-N5/1958/G3P[2] and RVA/Simiantc/ ZAF/SA11-N2/1958/G3P[2]. The genotype (N2) and sequence of genome segment 8 of RVA/Simian-tc/ZAF/ SA11-N2/1958/G3P[2] were identical to that of the bovine rotavirus O agent. Five novel amino acids were detected in minor population variants of three genome segments. Genome segment 1 (VP1) has a high nucleotide substitution rate, but the substitutions are synonymous. Distance matrices and Bayesian molecular clock phylogenetics showed that SA11-N2 is a reassortant containing genome segment 8 from the O agent, whereas SA11-N5 is a very close derivative of the prototype SA11-H96.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.titleWhole-genome consensus sequence analysis of a South African rotavirus SA11 sample reveals a mixed infection with two close derivatives of the SA11-H96 strainen_US
dc.typeArticleen_US
dc.contributor.researchID10997938 - Van Dijk, Alberdina Aike
dc.contributor.researchID21484317 - O'Neill, Hester Gertruida
dc.contributor.researchID22311890 - Mlera, Luwanika
dc.contributor.researchID22147322 - Jere, Khuzwayo C.


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