A novel reversed-phase LC method for quantitative detection of azithromycin in bulk drug and tablet formulations in various aqueous media
Abstract
A novel reversed-phase HPLC method with UV-detection for separation, identification and quantification
of azithromycin (AZH) was developed. The method was validated for the purpose of quantifying AZH in
stability, dissolution and solubility studies. The method was validated at two optimum wavelengths where
linear regression at both 205 and 210 nm, resulted in correlation coefficients of r2 = 0.9999. This HPLC
method proved to be superior to other published methods due to the specificity, resulting in less peak
interference and tailing. The pH of the mobile phase, as well as the ratio of buffer to organic component
included in the mobile phase, proved to be critical factors in the improved detection of AZH.
URI
http://hdl.handle.net/10394/14871http://www.ingentaconnect.com/contentone/govi/pharmaz/2012/00000067/00000012/art00004
https://doi.org/10.1691/ph.2012.2041
Collections
- Faculty of Health Sciences [2386]