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Reference gene selection for in vitro cell-free DNA analysis and gene expression profiling
(Elsevier, 2016)
Objectives: (i) To optimize cell-free DNA (cfDNA) and mRNA quantification using eight housekeeping genes
(HKGs), (ii) to determine if there is a difference in the occurrence of HKGs in the cfDNA andmRNA of normal cells
and ...
A quantitative assessment of cell-free DNA utilizing several housekeeping genes: measurements from four different cell lines
(Springer, 2016)
Quantitative real-time PCR (qPCR) is regularly used to quantify cell-free nucleic acids (cfNAs) in order to identify biomarkers for various pathologies. However, studies have shown notable housekeeping gene expression ...
An enquiry concerning the characteristics of cell-free DNA released by cultured cancer cells
(Springer, 2016)
Non-invasive screening that utilizes cell-free DNA (cfDNA) offers remarkable potential as a method for the early detection of genetic disorders and a wide variety of cancers. Unfortunately, one of the most prominent elements ...
Sequence analysis of cell-free DNA derived from cultured human bone osteosarcoma (143B) cells
(Sage, 2018)
The true importance of cell-free DNA in human biology, together with the potential scale of its clinical utility, is tarnished by a lack of understanding of its composition and origin. In investigating the cell-free DNA ...
Characterization of the cell-free DNA released by cultured cancer cells
(Elsevier, 2016)
The most prominent factor that delays the translation of cell-free DNA (cfDNA) analyses to clinical practice is the lack of knowledge regarding its origin and composition. The elucidation of the former is complicated by ...