Now showing items 1-4 of 4

    • Cell-free DNA: preanalytical variables 

      Bronkhorst, Abel Jacobus; Aucamp, Janine; Pretorius, Piet J. (Elsevier, 2015)
      Since the discovery of cell-free DNA (cfDNA) in human blood, most studies have focused on diagnostic and prognostic uses of these markers for solid tumors. Except for some prenatal tests and BEAMing, cfDNA analysis has not ...
    • Exposure to high levels of fumarate and succinate leads to apoptotic cytotoxicity and altered global DNA methylation profiles in vitro 

      Wentzel, Johannes F.; Lewies, Angelique; Bronkhorst, Abel J.; Van Dyk, Etresia; Du Plessis, Lissinda H.; Pretorius, Piet J. (Elsevier, 2017)
      In the Krebs cycle, succinate is oxidized to fumarate by succinate dehydrogenase (SDH), followed by the conversion of fumarate to malate by fumarate hydratase (FH). In cells with defective SDH and FH, the Krebs cycle is ...
    • Methodological variables in the analysis of cell-free DNA 

      Bronkhorst, Abel Jacobus; Aucamp, Janine; Pretorius, Piet J. (Springer, 2016)
      In recent years, cell-free DNA (cfDNA) analysis has received increasing amounts of attention as a potential non-invasive screening tool for the early detection of genetic aberrations and a wide variety of diseases, especially ...
    • Reference gene selection for in vitro cell-free DNA analysis and gene expression profiling 

      Bronkhorst, Abel Jacobus; Aucamp, Janine; Wentzel, Johannes F.; Pretorius, Piet J. (Elsevier, 2016)
      Objectives: (i) To optimize cell-free DNA (cfDNA) and mRNA quantification using eight housekeeping genes (HKGs), (ii) to determine if there is a difference in the occurrence of HKGs in the cfDNA andmRNA of normal cells and ...