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dc.contributor.authorMuleya, Victor
dc.contributor.authorWheeler, Janet I.
dc.contributor.authorFreihat, Lubna
dc.contributor.authorFreihat, David T.
dc.contributor.authorIrving, Helen R.
dc.contributor.authorRuzvidzo, Oziniel
dc.contributor.authorGehring, Chris
dc.identifier.citationMuleya, V. et al. 2014. Calcium is the switch in the moonlighting dual function of the ligand–activated receptor kinase phytosulfokine receptor 1. Cell Communication And Signaling, 12(60):1-5. [http://biosignaling.biomedcentral.com/articles]en_US
dc.description.abstractBackground: A number of receptor kinases contain guanylate cyclase (GC) catalytic centres encapsulated in the cytosolic kinase domain. A prototypical example is the phytosulfokine receptor 1 (PSKR1) that is involved in regulating growth responses in plants. PSKR1 contains both kinase and GC activities however the underlying mechanisms regulating the dual functions have remained elusive. Findings: Here, we confirm the dual activity of the cytoplasmic domain of the PSKR1 receptor. We show that mutations within the guanylate cyclase centre modulate the GC activity while not affecting the kinase catalytic activity. Using physiologically relevant Ca²⁺ levels, we demonstrate that its GC activity is enhanced over two-fold by Ca²⁺ in a concentration-dependent manner. Conversely, increasing Ca²⁺ levels inhibits kinase activity up to 500-fold at 100 nM Ca²⁺. Conclusions: Changes in calcium at physiological levels can regulate the kinase and GC activities of PSKR1. We therefore propose a functional model of how calcium acts as a bimodal switch between kinase and GC activity in PSKR1 that could be relevant to other members of this novel class of ligand-activated receptor kinases.en_US
dc.publisherBioMed Centralen_US
dc.subjectGuanylate cyclaseen_US
dc.titleCalcium is the switch in the moonlighting dual function of the ligand–activated receptor kinase phytosulfokine receptor 1en_US
dc.contributor.researchID22714839 - Ruzvidzo, Oziniel

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