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dc.contributor.authorConradie, Andelé M.
dc.contributor.authorPotgieter, Christiaan A.
dc.contributor.authorStassen, Liesel
dc.contributor.authorHuisman, Henk
dc.contributor.authorTheron, Jacques
dc.identifier.citationConradie, A.M. et al. 2016. Establishment of different plasmid only-based reverse genetics systems for the recovery of African horse sickness virus. Virology, 499:144-155. []en_US
dc.description.abstractIn an effort to simplify and expand the utility of African horse sickness virus (AHSV) reverse genetics, different plasmid-based reverse genetics systems were developed. Plasmids containing cDNAs corresponding to each of the full-length double-stranded RNA genome segments of AHSV-4 under control of a T7 RNA polymerase promoter were co-transfected in cells expressing T7 RNA polymerase, and infectious AHSV-4 was recovered. This reverse genetics system was improved by reducing the required plasmids from 10 to five and resulted in enhanced virus recovery. Subsequently, a T7 RNA polymerase expression cassette was incorporated into one of the AHSV-4 rescue plasmids. This modified 5-plasmid set enabled virus recovery in BSR or L929 cells, thus offering the possibility to generate AHSV-4 in any cell line. Moreover, mutant and cross-serotype reassortant viruses were recovered. These plasmid DNA-based reverse genetics systems thus offer new possibilities for investigating AHSV biology and development of designer AHSV vaccine strainsen_US
dc.subjectAfrican horse sickness virusen_US
dc.subjectReverse geneticsen_US
dc.subjectVirus rescueen_US
dc.subjectT7 RNA polymeraseen_US
dc.titleEstablishment of different plasmid only-based reverse genetics systems for the recovery of African horse sickness virusen_US
dc.contributor.researchID10085637 - Potgieter, Abraham Christiaan

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