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dc.contributor.authorMonapathi, M.E.
dc.contributor.authorBezuidenhout, C.C.
dc.contributor.authorRhode, O.H.J.
dc.date.accessioned2018-04-13T13:16:42Z
dc.date.available2018-04-13T13:16:42Z
dc.date.issued2018
dc.identifier.citationMonapathi, M.E. et al. 2018. Efflux pumps genes of clinical origin are related to those from fluconazole-resistant Candida albicans isolates from environmental water. Water science and technology, 77(4):899-908. [https://doi.org/10.2166/wst.2017.607]en_US
dc.identifier.issn0273-1223
dc.identifier.urihttp://hdl.handle.net/10394/26701
dc.identifier.urihttps://doi.org/10.2166/wst.2017.607
dc.identifier.urihttp://wst.iwaponline.com/content/77/4/899
dc.description.abstractEfflux pumps coded for by CDR1, CDR2, FLU1 and MDR1 genes could be responsible for the observed resistant phenotypes in azole-resistant Candida albicans from environmental water. This was demonstrated for clinical isolates. The aim of this study was to determine the presence and genetic similarity between efflux pump genes from clinical and environmental C. albicans isolates. Yeasts were isolated and identified using 26S rRNA gene sequencing. Disk diffusion tests were conducted. PCR was used to detect the presence of efflux genes. The fragments were sequenced and subjected to BLAST and subsequent phylogenetic analysis. Thirty seven C. albicans were identified from five selected rivers; Mooi River (19 isolates), Harts River (9 isolates), Marico River (5 isolates), Crocodile River (3 isolates) and Schoonspruit River (1 isolate). All the isolates were completely resistant to azoles. Efflux pump genes were detected in most (≥60%) of the isolates. Phylogenetic analysis showed high sequence similarity between sequences from environmental isolates and clinical isolates. Resistance to the azoles and the detection of efflux pump genes renders these antifungal agents ineffective. This is a major problem, particularly for the immune-compromised sector of the community of the North West Province and warrants further investigationen_US
dc.language.isoenen_US
dc.publisherIWA Publishingen_US
dc.subjectCandida albicansen_US
dc.subjectEfflux pump genesen_US
dc.subjectFluconazole resistanceen_US
dc.subjectPhylogenetic analysisen_US
dc.subjectYeast infectionsen_US
dc.titleEfflux pumps genes of clinical origin are related to those from fluconazole-resistant Candida albicans isolates from environmental wateren_US
dc.typeArticleen_US
dc.contributor.researchID12540110 - Bezuidenhout, Cornelius Carlos


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