Show simple item record

dc.contributor.authorClifford, Tom
dc.contributor.authorHowatson, Glyn
dc.contributor.authorConstantinou, Costas M.
dc.contributor.authorKeane, Karen M.
dc.contributor.authorWest, Daniel J.
dc.identifier.citationClifford, T. et al. 2017. The plasma bioavailability of nitrate and betanin from Beta vulgaris rubra in humans. European journal of nutrition, 56:1245-1254. []en_US
dc.identifier.issn1436-6215 (Online)
dc.description.abstractPurpose To evaluate the plasma bioavailability of betanin and nitric oxide (NOx) after consuming beetroot juice (BTJ) and whole beetroot (BF). BTJ and BF were also analysed for antioxidant capacity, polyphenol content (TPC) and betalain content. Methods Ten healthy males consumed either 250 ml of BTJ, 300 g of BF or a placebo drink, in a randomised, crossover design. Venous plasma samples were collected pre (baseline), 1, 2, 3, 5 and 8 h post-ingestion. Betanin content in BTJ, BF and plasma was analysed with reverse-phase high-performance liquid chromatography (HPLC) and mass spectrometry detection (LCMS). Antioxidant capacity was estimated using the Trolox equivalent antioxidant capacity (TEAC) and polyphenol content using Folin–Ciocalteu colorimetric methods [gallic acid equivalents (GAE)] and betalain content spectrophotometrically. Results TEAC was 11.4 ± 0.2 mmol/L for BTJ and 3.4 ± 0.4 μmol/g for BF. Both BTJ and BF contained a number of polyphenols (1606.9 ± 151 mg/GAE/L and 1.67 ± 0.1 mg/GAE/g, respectively), betacyanins (68.2 ± 0.4 mg/betanin equivalents/L and 19.6 ± 0.6 mg/betanin equivalents/100 g, respectively) and betaxanthins (41.7 ± 0.7 mg/indicaxanthin equivalents/L and 7.5 ± 0.2 mg/indicaxanthin equivalents/100 g, respectively). Despite high betanin contents in both BTJ (~194 mg) and BF (~66 mg), betanin could not be detected in the plasma at any time point post-ingestion. Plasma NOx was elevated above baseline for 8 h after consuming BTJ and 5 h after BF (P < 0.05). Conclusions These data reveal that BTJ and BF are rich in phytonutrients and may provide a useful means of increasing plasma NOx bioavailability. However, betanin, the major betalain in beetroot, showed poor bioavailability in plasmaen_US
dc.subjectNitric oxideen_US
dc.titleThe plasma bioavailability of nitrate and betanin from Beta vulgaris rubra in humansen_US
dc.contributor.researchID26084759 - Howatson, Glyn

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record