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dc.contributor.authorDu Preez, Ilse
dc.contributor.authorBeukes, Derylize
dc.contributor.authorLoots, Du Toit
dc.date.accessioned2019-03-25T07:00:18Z
dc.date.available2019-03-25T07:00:18Z
dc.date.issued2019
dc.identifier.citationDu Preez, I. et al. 2019. Fatty acid metabolome extraction from mycobacterial cells for GC-MS metabolomics analysis. (In Baidoo, E.E.K., ed. Microbial metabolomics: methods and protocols). Methods in molecular biology, 1859:111-120. [https://doi.org/10.1007/978-1-4939-8757-3_5]en_US
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029 (Online)
dc.identifier.issn978-1-4939-8756-6
dc.identifier.issn978-1-4939-8757-3 (Online)
dc.identifier.urihttp://hdl.handle.net/10394/32014
dc.identifier.urihttps://link.springer.com/protocol/10.1007/978-1-4939-8757-3_5
dc.identifier.urihttps://doi.org/10.1007/978-1-4939-8757-3_5
dc.description.abstractMetabolomics is becoming an increasingly popular research tool for identifying new biomarkers, which can, among other applications, be applied to elucidate various microbial growth and virulence mechanisms. Since the lipid composition of numerous microorganisms are unique and characteristic of the particular species, and in many instances also associated with several of their growth and virulence features, we developed a method for extracting the total free fatty acid metabolome from mycobacterial cells, in order to better characterize these using a gas chromatography–mass spectrometry (GC-MS) metabolomics approach. The described method can be considered an optimized Bligh–Dyer approach, since it uses the traditional solvents; chloroform, methanol and water, in a ratio of 1:2:1. However, due to the robust cell walls associated with mycobacteria, and many other microorganisms, the method was adapted to include a step which allows for the physical disruption of the cells using a vibration mill, which dramatically increases the efficiency of this approach. Hereafter, the organic phase is collected, dried, and methylated (as a derivatization step), prior to GC-MS analysesen_US
dc.language.isoenen_US
dc.publisherHumanaen_US
dc.subjectFatty acid metabolome extractionen_US
dc.subjectGas chromatography–mass spectrometryen_US
dc.subjectMetabolomicsen_US
dc.subjectMycobacteriaen_US
dc.titleFatty acid metabolome extraction from mycobacterial cells for GC-MS metabolomics analysisen_US
dc.typeBook chapteren_US
dc.contributor.researchID10799508 - Loots, Du Toit
dc.contributor.researchID20026471 - Du Preez, Ilse


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