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dc.contributor.authorDe Menezs, Elaine C. Soares
dc.contributor.authorElson, Joanna L.
dc.contributor.authorBrown, Audrey E.
dc.contributor.authorBowman, Simon J.
dc.contributor.authorMirza, Kamran
dc.identifier.citationDe Menezes, E.C.S. et al. 2018. Mitochondrial DNA copy number is not associated with fatigue status in Primary Sjögren’s Syndrome. Fatigue: biomedicine, health and behavior, 6(3):123-131. []en_US
dc.identifier.issn2164-1862 (Online)
dc.description.abstractBackground: Primary Sjögren’s syndrome (pSS) is a heterogeneous disease characterized by lymphocytic infiltrates to the exocrine glands, causing sicca symptoms and other manifestations. Fatigue is one of the most prominent symptom in pSS; up to 70% suffer from chronic fatigue. Fatigue has been shown to be common and severe in patients suffering from primary mitochondrial DNA (mtDNA) disease. In a number of chronic diseases, mitochondrial DNA copy number (mtDNAcn) has been reported to be altered. Purpose: The aim of the study was to examine if mtDNAcn was altered in fatigued versus non-fatigued pSS. Methods: We quantified mtDNAcn using quantitative polymerase chain reaction (qPCR) with mitochondrial ND2 as a target gene normalized to nuclear HβB (mtDNA:nDNA). Results: In 204 participants, 108 fatigued and 96 non-fatigued, relative mtDNAcn did not distinguish fatigue status (p = 0.7) nor was it correlated with severity of fatigue (p = 0.21). Conclusions: MtDNAcn is not altered with fatigue status in blood in pSS. Our analysis suggests that when conducting mtDNAcn analysis with large sample numbers over multiple days a two-way-ANOVA should be used in preference to a one-way-ANOVA or t-test to allow detection of batch effectsen_US
dc.publisherTaylor & Francisen_US
dc.subjectMitochondrial DNA copy number (mtDNAcn)en_US
dc.subjectPrimary Sjögren’s Syndromeen_US
dc.titleMitochondrial DNA copy number is not associated with fatigue status in Primary Sjögren’s Syndromeen_US
dc.contributor.researchID24952338 - Elson, Joanna L.

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