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dc.contributor.authorPhilippe, Charlotte
dc.contributor.authorBrendonck, Luc
dc.contributor.authorGregoir, Arnoit F.
dc.contributor.authorThoré, Eli S.J.
dc.contributor.authorDe Boeck, Gudrun
dc.identifier.citationPhilippe, C. et al. 2018. Protocol for acute and chronic ecotoxicity testing of the turquoise killifish Nothobranchius furzeri. Journal of visualized experiments, 134: Article no e57308. []en_US
dc.identifier.issn1940-087X (Online)
dc.description.abstractThe killifish Nothobranchius furzeri is an emerging model organism in the field of ecotoxicology and its applicability in acute and chronic ecotoxicity testing has been demonstrated. Overall, the sensitivity of the species to toxic compounds is in the range with, or higher than, that of other model species. This work describes protocols for acute, chronic, and multigenerational bioassays of single and combined stressor effects on N. furzeri. Due to its short maturation time and life-cycle, this vertebrate model allows the study of endpoints such as maturation time and fecundity within four months. Transgenerational full life-cycle exposure trials can be performed in as little as 8 months. Since this species produces eggs that are drought-resistant and remain viable for years, the on-site culture of the species is not needed but individuals can be recruited when required. The protocols are designed to measure life-history traits (mortality, growth, fecundity, weight) and critical thermal maximumen_US
dc.titleProtocol for acute and chronic ecotoxicity testing of the turquoise killifish Nothobranchius furzerien_US
dc.contributor.researchID29898382 - Brendonck, Luc Gerard Eric

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