In-vitro cytotoxicity of various Siphonochilus aethiopicus (Schweinf.) B.L. Burtt extracts in combination with selected tableting excipients
Date
2019Author
Erasmus, Mandi
Du Plessis, Lissinda H.
Viljoen, Joe M.
Metadata
Show full item recordAbstract
Objectives To investigate the cytotoxic potential of S. aethiopicus extracts in
combination with chitosan and Pharmacel 101, on two cell lines.
Methods Extracts were chemically characterised utilising UPLC-Q-TOF/MS, followed by determination of cell viability and membrane integrity.
Key findings Ethanol (EtOH) and diethyl ether (DiEt) extracts contained significant quantities of all chosen biomarker molecules; however, only two were scarcely quantifiable in aqueous extracts. Aqueous extracts did not induce any
cytotoxic effects, whereas EtOH and DiEt extracts caused concentrationdependent decreases in cell viability and membrane integrity loss in both cell
lines. Ensuing exposure to EtOH extracts at 50, 100 and 150 lg/ml, HepG2 cells
were considered 15.5%, 12.5% and 32.8% apoptotic, whereas DiEt extracts
caused 4.5%, 13.5% and 33.9% apoptotic cells. Exposure to EtOH and DiEt
extracts at 50 lg/ml ensued in 20.2% and 21.3% apoptosis in Caco-2 cells;
100 lg/ml induced apoptosis in 19.9% and 10.3% of Caco-2 cells; whereas exposure to 150 lg/ml EtOH extracts caused 12.6% apoptosis compared to 11.7%
induced by the DiEt extract.
Conclusions None of the excipients caused any significantly altered cellular
effects, indicating little chance for physicochemical interactions. Aqueous extracts
did not possess any cytotoxic properties. However, it is clear that organic extracts
caused apoptotic and necrotic cell death
URI
http://hdl.handle.net/10394/33354https://onlinelibrary.wiley.com/doi/pdf/10.1111/jphp.13160
https://doi.org/10.1111/jphp.13160
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- Faculty of Health Sciences [2377]