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dc.contributor.authorTakyi-Williams, John
dc.contributor.authorErasmus, Linné
dc.contributor.authorHayeshi, Rose
dc.contributor.authorGrobler, Anne
dc.date.accessioned2020-04-02T06:59:46Z
dc.date.available2020-04-02T06:59:46Z
dc.date.issued2020
dc.identifier.citationTakyi-Williams, J. et al. 2020. Development and validation of an LC-MS/MS method for the quantification of goserelin in a Pheroid (R) formulation, in simulated intestinal fluid. Journal of pharmaceutical and biomedical analysis, 180: #113044. [https://doi.org/10.1016/j.jpba.2019.113044]en_US
dc.identifier.issn0731-7085
dc.identifier.urihttp://hdl.handle.net/10394/34489
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0731708519324446
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2019.113044
dc.description.abstractThe purpose of this reported study was to develop and validate an LC-MS/MS method for the quantification of goserelin in a Pheroid (R) formulation simulated intestinal fluid. Biopharmaceuticals are formulated in drug delivery systems to improve their gastrointestinal stability. Goserelin, a peptide drug was formulated in Pheroid (R) delivery system and its gastrointestinal stability assessed using simulated intestinal fluid, which required an assay to determine the varying amounts of goserelin remaining after a specific time. Several extraction methods and solvents investigated to extract goserelin from complex matrix led to either poor recovery, peak shape or high background interference. A rapid gradient reversed-phase method coupled to tandem mass spectrometry detection was optimized for the separation and quantification of the extracted peptide. A simple, reproducible and good recovery extraction procedure for goserelin quantification was achieved through simultaneous acetonitrile protein precipitation and water-saturated n-butanol liquid-liquid extraction with water dilution. The method was found to be rapid, specific, precise and accurate, and successfully applied to determine goserelin remaining content in a simulated intestinal fluid, with potential use in other lipid-based formulation evaluated in simulated intestinal fluids. (C) 2019 Elsevier B.V. All rights reserveden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectPheroid (R) formulationen_US
dc.subjectGoserelinen_US
dc.subjectLC-MS/MSen_US
dc.subjectPeptideen_US
dc.subjectSimulated intestinal fluiden_US
dc.subjectMethod validationen_US
dc.titleDevelopment and validation of an LC-MS/MS method for the quantification of goserelin in a Pheroid (R) formulation, in simulated intestinal fluiden_US
dc.typeArticleen_US
dc.contributor.researchID26419904 - Hayeshi, Rose Khavogoi
dc.contributor.researchID11008857 - Grobler, Anne Frederica
dc.contributor.researchID30562872 - Takyi-Williams, John
dc.contributor.researchID23389907 - Erasmus, Linné


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