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dc.contributor.authorDu Plessis, Lissinda
dc.contributor.authorLaubscher, Petrus
dc.contributor.authorJooste, Jacques
dc.contributor.authorDu Plessis, Johan
dc.contributor.authorFranken, Anja
dc.contributor.authorVan Aarde, Nico
dc.contributor.authorEloff, Fritz
dc.identifier.citationDu Plessis, L. et al. 2010. Flow cytometric analysis of the oxidative status in human peripheral blood mononuclear cells of workers exposed to welding fumes. Journal of occupational and environmental hygiene, 7(6):367-374. []en_US
dc.identifier.issn1545-9632 (Online)en_US
dc.description.abstractFlow cytometry is a simple analytical technique that identifies, counts, and characterizes cells. The oxidative status of cells is influenced by many exogenous sources, such as occupational exposure to welding fumes. This study evaluated flow cytometry as a method to determine the oxidative status of male welders (n = 15) occupationally exposed to welding fumes. Flow cytometric analysis of reactive oxygen species (ROS) was carried out in peripheral blood mononuclear cells (PBMC) by using the probe 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA). Lipid peroxidation was measured by the decrease of fluor-DHPE fluorescence and intracellular glutathione (GSH) levels by using mercury orange. All of the parameters were also observed under a confocal microscope. The oxidative stress ratio was calculated from the oxidative damage and the antioxidant capacity to give an accurate account of the cellular oxidative status. ROS and lipid peroxidation levels were elevated by ∼ 87% and ∼ 96%, respectively, and GSH levels lowered ∼ 96% in PBMC of workers exposed to welding fumes compared with non-exposed controls. The oxidative stress ratio was significantly higher (p < 0.001) in the exposed group. Flow cytometry can be useful for the measurement of cellular oxidative stress in somatic cells of workers exposed to welding fumes and other occupational settings. Calculating an oxidative stress index may be useful in predicting disease outcomes and whether preventative control measures are efficient
dc.publisherTaylor & Francisen_US
dc.subjectFlow cytometry
dc.subjectLipid peroxidation
dc.subjectReactive oxygen species
dc.subjectWelding fume
dc.titleFlow cytometric analysis of the oxidative status in human peripheral blood mononuclear cells of workers exposed to welding fumesen_US
dc.contributor.researchID11948388 - Du Plessis, Lissinda Hester
dc.contributor.researchID10101268 - Du Plessis, Johannes Lodewykus
dc.contributor.researchID10060790 - Eloff, Frederik Christoffel
dc.contributor.researchID10058028 - Van Aarde, Michiel Nicolaas
dc.contributor.researchID12776998 - Franken, Anja
dc.contributor.researchID10057773 - Laubscher, Petrus Johannes
dc.contributor.researchID12860492 - Jooste, Jacques

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